ABSTRACT
Abstract Vitamin D plays a vital role in calcium and phosphate balance and bone structure. In recent times vitamin D deficiency has become a well-known epidemic. The current study shows vitamin D deficiency causes various types of cancer along with heart disease. The current diet has removed most of the best sources of vitamin D. Healthy bones and teeth need vitamin D. Most of the low fat diets have removed vitamin D from the diet. Vitamin D has to be consumed with fatty meal. Food items such as dairy products are hardly fortified with vitamin D in India. Subclinical vitamin D deficiency is extremely prevalent in both urban and rural settings. In USA and Canada, in spite of having food fortification techniques still vitamin D deficiency prevails. In this paper various case studies related to vitamin D deficiency in relation to cancer have been reviewed. A proper diet to overcome this problem have been suggested along with some preventive measures
ABSTRACT
Polymerase chain reaction (PCR) is the most sensitive test to diagnose HIV-1 infection among infants born to HIV seropositive mothers. The purpose of this study was to evaluate the use of dried blood spot (DBS) specimens for PCR and to compare it with whole-blood stored in tubes for HIV-1 DNA PCR. Five hundred and seventy-seven whole-blood infant samples were tested using HIV-1 qualitative in-house nested DNA PCR. Three hundred and fifty-nine samples were from infants at 48 hours of birth and 218 samples at second month. All positive samples tested from whole-blood and every fifth negative sample were coated onto filter paper. DNA was extracted from the filter paper and was amplified using in-house nested PCR. Among the whole-blood samples tested using HIV-1 DNA PCR, 19 of 359 (5.29%) samples were HIV-1 positive and 340 (94.7%) were negative at 48 hours of birth. At second month, 19 (8.7%) of the 218 samples were positive and 199 (91.2%) were negative. Using dried filter paper, 18 samples (95%) tested positive from 19 positive samples (using whole-blood) and 1 tested negative at 48 hours of birth. The 68 negative samples tested using whole-blood were also negative in the DBS test (sensitivity 95% and specificity 100%). At second month, 19 were positive and 40 samples (every fifth sample of 199) were negative (sensitivity and specificity, 100%). PCR performed using DNA extracted from filter paper permits the diagnosis of HIV-1 infection among infants born to HIV-1 seropositive mothers. This assay is simple, rapid, sensitive and specific and can be used in resource limited settings.